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Western blot analysis using NF-κB p65 mouse mAb against Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.
Western blot analysis using NF-κB p65 mouse mAb against RSC-96(1),KO-SF(2),NIH/3T3(3),NRK(4),C2C12(5),C6(6),81505(7) cell lysate.
Immunohistochemical analysis of paraffin-embedded Mouse kidney(A) Mouse liver (B) using NF-κB p65 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat spleen(A) Rat liver (B) using NF-κB p65 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit spleen(A) Rabbit Rabbit liver(B) using NF-κB p65 mouse mAb with DAB staining.
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Mouse Monoclonal Antibody to NF-κB p65

  • 货号:

    20406
  • 别名:

    NFkappaB p65; p65; NFKB3; RELA
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC
  • 反应种属:

    Human,Mouse,Rat,Rabbit,Monkey
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q04206
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to NF-κB p65

Description

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity.

References

1. Nature. 1997 Aug 7;388(6642):548-54.
2. Cell. 1998 Dec 11;95(6):749-58.
3. J Biol Chem. 2000 Jun 16;275(24):18180-7.

Specification

Aliases NFkappaB p65; p65; NFKB3; RELA
Entrez GeneID 5970
Swissprot Q04206
clone 6H7
WB Predicted band size 65kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat,Rabbit,Monkey
Immunogen Purified recombinant fragment of human NF-κB p65 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500 - 1/2000
IHC 1/100 - 1/500
ELISA 1/10000