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All lanes : Anti-PRKAA1 Antibody at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: MDA-MB-468 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-PRKAA1 Antibody (Ascites) at 1:8000 dilution + K562 whole cell lysate Secondary Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64009 Da Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-PRKAA1 Antibody (Ascites) at 1:4000 dilution + MDA-MB-453 whole cell lysate Secondary Goat Anti-mouse IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 64009 Da Blocking/Dilution buffer: 5% NFDM/TBST.
PRKAA1 Monoclonal( Ascites) (Cat. #P34236) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRKAA1 Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.

Mouse Monoclonal Antibody to PRKAA1

  • 货号:

    P34236
  • 别名:

    5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q13131
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to PRKAA1

Description

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Specification

Aliases 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1
Entrez GeneID 5562
Swissprot Q13131
WB Predicted band size 64.0kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This PRKAA1 Monoclonal antibody was raised using purified His-tagged recombinant human PRKAA1.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/1000-1/5000
IHC 1/100-1/500