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Western blot analysis using MAP4K4 mouse mAb against Jurkat (1),PC-12(2), Jurkat (3) ,HepG2(4) ,K652(5) lysate.
Immunofluorescence analysis of hela cells using MAP4K4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of NIH3T3 cells using MAP4K4 mouse mAb (green) and negative control (red).
Flow cytometric analysis of hela cells using MAP4K4 mouse mAb (green) and negative control (red).
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Mouse Monoclonal Antibody to MAP4K4
-
货号：
20234 -
别名：
HGK; NIK -
应用：
WB,IF,FCM -
反应种属：
Human,Mouse -
抗体类型：
Primary antibody -
Swissprot：
O95819 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to MAP4K4
Description |
|---|
MAP4K4: mitogen-activated protein kinase kinase kinase kinase 4. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. |
References |
|---|
| 1. Mol Cell Biol. 2000 Mar;20(5):1537-45. 2. Curr Biol. 2002 Apr 16;12(8):622-31. 3. J Biol Chem. 2007 Mar 16;282(11):7783-9. |
Specification |
|
|---|---|
| Aliases | HGK; NIK |
| Entrez GeneID | 9448 |
| Swissprot | O95819 |
| clone | 4H9E7 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of MAP4K4 (aa400-500) expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/50 - 1/200 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Western blot analysis using MAP4K4 mouse mAb against Jurkat (1),PC-12(2), Jurkat (3) ,HepG2(4) ,K652(5) lysate.
- Immunofluorescence analysis of hela cells using MAP4K4 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of NIH3T3 cells using MAP4K4 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of hela cells using MAP4K4 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#20234
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