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Western blot analysis of eIF4E (Phospho-S209) in (1) HEK293 lysates; (2) mouse spleen lysates using Phospho-eIF4E (Ser209) antibody.
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Phospho-eIF4E (S209) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Rabbit Polyclonal Antibody to Phospho-eIF4E (Ser209)

  • 货号:

    P20237
  • 别名:

    EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IP,IHC,IF
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P06730
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Polyclonal Antibody to Phospho-eIF4E (Ser209)

Description

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Specification

Aliases EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
Entrez GeneID 1977
Swissprot P06730
WB Predicted band size Calculated MW: 25 kDa; Observed MW: 25 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen A synthesized peptide derived from human Phospho-eIF4E (S209)
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IP 1/10-1/20
IHC 1/50-1/100
IF 1/50-1/200

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P20237