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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using NEFM mouse mAb (green) and negative control (red).
Flow cytometric analysis of Raji cells using NEFM mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to NEFM

  • 货号:

    31145
  • 别名:

    NFM; NEF3; NF-M
  • 产品上架时间:

    2024-01-01
  • 应用:

    IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P07197
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to NEFM

Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

References

1.J Neuropathol Exp Neurol. 2004 Jul;63(7):759-74.
2.Neurosci Lett. 2003 Nov 13;351(2):125-9.

Specification

Aliases NFM; NEF3; NF-M
Entrez GeneID 4741
Swissprot P07197
clone 1B6B12
WB Predicted band size 102.5kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human NEFM (AA: 779-916) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

IF 1/50 - 1/200
FCM 1/200 - 1/400
ELISA 1/10000