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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.
Flow cytometric analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using RF1 mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to RF1

  • 货号:

    30965
  • 别名:

    ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P62495
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to RF1

Description

This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.

References

1.Nucleic Acids Res. 2013 Apr;41(8):4573-86.
2.Protein Sci. 2012 Jun;21(6):896-903.

Specification

Aliases ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1
Entrez GeneID 2107
Swissprot P62495
clone 4F9H12
WB Predicted band size 49kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
FCM 1/200 - 1/400
ELISA 1/10000