提醒成功
搜索
Mouse Monoclonal Antibody to RF1
-
货号:
30965 -
别名:
ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1 -
产品上架时间:
2024-01-01 -
应用:
WB,FCM -
反应种属:
Human -
抗体类型:
Primary antibody -
Swissprot:
P62495 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180
Mouse Monoclonal Antibody to RF1
Description |
---|
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. |
References |
---|
1.Nucleic Acids Res. 2013 Apr;41(8):4573-86. 2.Protein Sci. 2012 Jun;21(6):896-903. |
Specification |
|
---|---|
Aliases | ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1 |
Entrez GeneID | 2107 |
Swissprot | P62495 |
clone | 4F9H12 |
WB Predicted band size | 49kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
---|---|
WB | 1/500 - 1/2000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.
- Flow cytometric analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of HepG2 cells using RF1 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30965
相关产品