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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using RAD21 mouse mAb against Hela (1), HEK293 (2), K562 (3), C6 (4), *** (5), and COS7 (6) cell lysate.

Immunofluorescence analysis of Hela cells using RAD21 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using RAD21 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded human brain tissues using RAD21 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using RAD21 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to RAD21

货号：
30969
别名：
HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
产品上架时间：
2024-01-01
应用：
WB,IHC,IF,FCM
反应种属：
Human,Rat,Monkey
抗体类型：
Primary antibody
Swissprot：
O60216
规格：

100μl
50μl
20μl
数量：

-

+
说明书：

目录价¥2180

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Mouse Monoclonal Antibody to RAD21

Description
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Description

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

References
1.Breast Cancer Res. 2012 Apr 26;14(2):R69. 2.Breast Cancer Res. 2011 Jan 21;13(1):R9.

Specification
Aliases	HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
Entrez GeneID	5885
Swissprot	O60216
clone	1B6D1
WB Predicted band size	71.7kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Rat,Monkey
Immunogen	Purified recombinant fragment of human RAD21 (AA: 287-403) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide