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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.
Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Raji cells using HIST2H4A(20Me) mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.

Mouse Monoclonal Antibody to HIST2H4A(20Me)

  • 货号:

    30885
  • 别名:

    H4; H4/n; H4F2; H4FN; FO108; HIST2H4
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P62805
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to HIST2H4A(20Me)

Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

References

1.J Virol. 2011 Dec;85(24):13234-52.
2.Mol Cell Biol. 2003 Feb;23(4):1460-9.

Specification

Aliases H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Entrez GeneID 8370
Swissprot P62805
clone 3E7H2
WB Predicted band size 11.4kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Synthesized peptide of human HIST2H4A ( AA: GGAKRHRK(Me)VLRDNIQ ).
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000