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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Immunofluorescence analysis of HeLa cells using SK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of HeLa cells using SK2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using SK2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using SK2 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to SK2

  • 货号:

    30888
  • 别名:

    SPHK2; SK-2; SPK 2; SPK-2
  • 产品上架时间:

    2024-01-01
  • 应用:

    IHC,IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q9NRA0
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to SK2

Description

This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

References

1.Tumour Biol. 2014 Jan;35(1):363-8.
2.Electrophoresis. 2011 Jun;32(13):1742-9.

Specification

Aliases SPHK2; SK-2; SPK 2; SPK-2
Entrez GeneID 56848
Swissprot Q9NRA0
clone 9C5E1
WB Predicted band size 69.2kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Synthesized peptide of human SK2 (AA: 36-52).
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

IHC 1/200 - 1/1000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000