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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), SK-Br-3 (3), Hela (4), Lncap (5), and HepG2 (6) cell lysate.

Immunofluorescence analysis of MCF-7 cells using CK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using CK5 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to CK5

货号：
30891
别名：
KRT5; K5; DDD; DDD1; EBS2; KRT5A
产品上架时间：
2024-01-01
应用：
WB,IHC,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P13647
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Mouse Monoclonal Antibody to CK5

Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

References
1.Horm Cancer. 2013 Feb;4(1):36-49. 2.Breast Cancer Res Treat. 2011 Jul;128(1):45-55.

Specification
Aliases	KRT5; K5; DDD; DDD1; EBS2; KRT5A
Entrez GeneID	3852
Swissprot	P13647
clone	2C8E4
WB Predicted band size	62.4kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human CK5 (AA: 258-357) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide