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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using CDH16 mouse mAb against A431 (1), Jurkat (2) cell lysate.
Immunofluorescence analysis of A431 cells using CDH16 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of A431 cells using CDH16 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human kidney tissues using CDH16 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to CDH16

  • 货号:

    30659
  • 别名:

    KSP-Cadherin
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    O75309
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to CDH16

Description

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

References

1. Br J Cancer. 2005 Jun 6;92(11):2010-7.
2. Am J Physiol. 1999 Jul;277(1 Pt 2):F146-56.

Specification

Aliases KSP-Cadherin
Entrez GeneID 1014
Swissprot O75309
clone 6H6E2
WB Predicted band size 90kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human CDH16 (AA: EX(558-721)) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000