ACLY[30437]
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.
Western blot analysis using ACLY mouse mAb against PC-12(1)Raw264.7(2)NIH/3T3(3)NRK(4)C6(5)F9(6)COS-7(7)CHO3D10(8)cell lysate.
Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence analysis of NIH/3T3 cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ACLY mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using ACLY mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse liver(A) Mouse kidney(B) using ACLY mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat liver(A) Rat kidney(B) using ACLY mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit liver(A) Rabbit kidney(B) using ACLY mouse mAb with DAB staining.

Mouse Monoclonal Antibody to ACLY

  • 货号:

    30437
  • 别名:

    ACL; ATPCL; CLATP
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human,Mouse,Rat,Monkey,Rabbit
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P53396
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to ACLY

Description

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.


References

1.J Biol Chem. 2010 Oct 15;285(42):32606-15.
2.Int J Cancer. 2010 May 15;126(10):2282-95.

Specification

Aliases ACL; ATPCL; CLATP
Entrez GeneID 47
Swissprot P53396
clone 5F8D11
WB Predicted band size 125kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat,Monkey,Rabbit
Immunogen Purified recombinant fragment of human ACLY (AA: 306-502 ) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/100 - 1/500
IF 1/50 - 1/500
FCM 1/200 - 1/400
ELISA 1/10000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#30437