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Mouse Monoclonal Antibody to ACLY
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Mouse Monoclonal Antibody to ACLY
Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.
Mouse Monoclonal Antibody to ACLY
Western blot analysis using ACLY mouse mAb against PC-12(1)Raw264.7(2)NIH/3T3(3)NRK(4)C6(5)F9(6)COS-7(7)CHO3D10(8)cell lysate.
Mouse Monoclonal Antibody to ACLY
Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Mouse Monoclonal Antibody to ACLY
Immunofluorescence analysis of NIH/3T3 cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Mouse Monoclonal Antibody to ACLY
Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple).
Mouse Monoclonal Antibody to ACLY
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ACLY mouse mAb with DAB staining.
Mouse Monoclonal Antibody to ACLY
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using ACLY mouse mAb with DAB staining.
Mouse Monoclonal Antibody to ACLY
Immunohistochemical analysis of paraffin-embedded Mouse liver(A) Mouse kidney(B) using ACLY mouse mAb with DAB staining.
Mouse Monoclonal Antibody to ACLY
Immunohistochemical analysis of paraffin-embedded Rat liver(A) Rat kidney(B) using ACLY mouse mAb with DAB staining.
Mouse Monoclonal Antibody to ACLY
Immunohistochemical analysis of paraffin-embedded Rabbit liver(A) Rabbit kidney(B) using ACLY mouse mAb with DAB staining.
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY
Mouse Monoclonal Antibody to ACLY

Mouse Monoclonal Antibody to ACLY

  • 货号:

    30437
  • 别名:

    ACL; ATPCL; CLATP
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human,Mouse,Rat,Monkey,Rabbit
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P53396
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to ACLY

Description

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.


References

1.J Biol Chem. 2010 Oct 15;285(42):32606-15.
2.Int J Cancer. 2010 May 15;126(10):2282-95.

Specification

Aliases ACL; ATPCL; CLATP
Entrez GeneID 47
Swissprot P53396
clone 5F8D11
WB Predicted band size 125kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat,Monkey,Rabbit
Immunogen Purified recombinant fragment of human ACLY (AA: 306-502 ) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/100 - 1/500
IF/ICC 1/50 - 1/500
FCM 1/200 - 1/400
ELISA 1/10000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#30437