KEAP1[30431]
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using KEAP1 mouse mAb against NIH3T3 (1), and A549 (2) cell lysate.
Immunofluorescence analysis of HeLa cells using KEAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of HepG2 cells using KEAP1 mouse mAb (green) and negative control (purple).

Mouse Monoclonal Antibody to KEAP1

  • 货号:

    30431
  • 别名:

    INrf2; KLHL19
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF,FCM
  • 反应种属:

    Human,Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q14145
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to KEAP1

Description

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.





References

1.Cell Signal. 2010 Nov;22(11):1645-54.
2.Mol Cancer Ther. 2010 Feb;9(2):336-46.

Specification

Aliases INrf2; KLHL19
Entrez GeneID 9817
Swissprot Q14145
clone 1F10B6
WB Predicted band size 69.7kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse
Immunogen Purified recombinant fragment of human KEAP1 (AA: 380-624) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

IF 1/50
FCM 1/200 - 1/400
ELISA 1/10000