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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate.
Flow cytometric analysis of Jurkat cells using GPC3 mouse mAb (green) and negative control (red).
Flow cytometric analysis of NIH/3T3 cells using GPC3 mouse mAb (green) and negative control (red).
Flow cytometric analysis of C6 cells using GPC3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using GPC3 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using GPC3 mouse mAb with DAB staining.
HOT

Mouse Monoclonal Antibody to GPC3

  • 货号:

    30372
  • 别名:

    SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
  • 产品上架时间:

    2024-05-03
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P51654
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to GPC3

Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.

References

1. Int J Cancer. 2010 Mar 15;126(6):1291-301.
2. Cancer Biol Ther. 2009 Dec;8(24):2329-38.

Specification

Aliases SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Entrez GeneID 2719
Swissprot P51654
clone 9C2
WB Predicted band size 65.5kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen Purified recombinant fragment of human GPC3 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000