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Mouse Monoclonal Antibody to AURKA
-
货号:
30160 -
别名:
AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; MGC34538; AURKA -
应用:
WB,IF,FCM -
反应种属:
Human,Mouse,Rat,Monkey -
抗体类型:
Primary antibody -
Swissprot:
O14965 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180
Mouse Monoclonal Antibody to AURKA
Description |
|---|
Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. |
References |
|---|
| 1. Cell Cycle. 2008 Nov 15;7(22):3525-33. 2. Oncogene. 2008 Nov 20;27(51):6539-49. |
Specification |
|
|---|---|
| Aliases | AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; MGC34538; AURKA |
| Entrez GeneID | 6790 |
| Swissprot | O14965 |
| clone | 1F8 |
| WB Predicted band size | 48kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human AURKA expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF/ICC | 1/50 - 1/500 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
- Western blot analysis using AURKA mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate.
- Western blot analysis using AURKA mouse mAb against F9 cell lysate.
- Immunofluorescence analysis of Hela cells using AURKA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
- Immunofluorescence analysis of NIH/3T3 cells using AURKA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
- Flow cytometric analysis of K562 cells using AURKA mouse mAb (green) and negative control (purple).
- Flow cytometric analysis of NIH/3T3 cells using AURKA mouse mAb (green) and negative control (red).
- Flow cytometric analysis of PC-12 cells using AURKA mouse mAb (green) and negative control (red).
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30160
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