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Mouse Monoclonal Antibody to Pirh2
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货号:
30005 -
别名:
ARNIP; CHIMP; RNF199; RCHY1 -
应用:
WB,IHC,ICC,FCM -
反应种属:
Human,Rat -
抗体类型:
Primary antibody -
规格:
-
数量:
-+ -
说明书:
目录价¥2180
Mouse Monoclonal Antibody to Pirh2
Description |
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Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function. |
References |
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1. Biochem Biophys Res Commun. 2007 Dec 14;364(2):344-50. 2. J Natl Cancer Inst. 2004 Nov 17;96(22):1718-21. 3. Exp Cell Res. 2006 Oct 15;312(17):3370-8. 4. Cell. 2003 Mar 21;112(6):779-91. |
Specification | |
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Aliases | ARNIP; CHIMP; RNF199; RCHY1 |
Entrez GeneID | 25898 |
Swissprot | Q96PM5 |
clone | 1H10 |
WB Predicted band size | 30kDa; 60kDa (homodimer) |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Rat |
Immunogen | Purified recombinant fragment of human Pirh2 expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application | |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
ICC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
- Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 mouse mAb
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30005
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