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Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Immunofluorescence analysis of A549 cells using RAB25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of NIH/3T3 cells using Rab25 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
HOT

Mouse Monoclonal Antibody to Rab25

  • 货号:

    20430
  • 别名:

    CATX-8; RAB25
  • 产品上架时间:

    2024-11-05
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human,Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P57735
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to Rab25

Description

Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.

References

1. JR Goldenring, KR Shen, HD Vaughan. et al. J. Biol. Chem,1993,268(25):18419-18422
2. Xiaoye W, Ravindra K, Jennifer N. et al. J. Biol. Chem,2000,275(37):29138-29146

Specification

Aliases CATX-8; RAB25
Entrez GeneID 57111
Swissprot P57735
clone 3F12
WB Predicted band size 24kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse
Immunogen Purified recombinant fragment of Rab25 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000