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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using KAT2B mouse mAb against C2C12 (1), COS7 (2), HepG2 (3), HCT116 (4), A431 (5), LNCAP (6), NIH/3T3 (7), and F9 (8) cell lysate.
Immunofluorescence analysis of Hela cells using KAT2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of MOLT4 cells using KAT2B mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using KAT2B mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using KAT2B mouse mAb with DAB staining.

Mouse Monoclonal Antibody to KAT2B

  • 货号:

    31630
  • 别名:

    CAF; PCAF; P/CAF
  • 产品上架时间:

    2024-01-01
  • 应用:

    IHC,IF,FCM
  • 反应种属:

    Human,Mouse,Rat,Monkey
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q92831
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to KAT2B

Description

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

References

1.Cell Death Dis. 2016 Oct 6;7(10):e2400.
2.J Biol Chem. 2016 Jul 1;291(27):14363-72.

Specification

Aliases CAF; PCAF; P/CAF
Entrez GeneID 8850
Swissprot Q92831
clone 7D12C8
WB Predicted band size 93kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat,Monkey
Immunogen Purified recombinant fragment of human KAT2B (AA: 1-142) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

IHC 1/200 - 1/1000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000