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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis of Hela cells using EHMT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of HL-60 cells using EHMT2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using EHMT2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using EHMT2 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to EHMT2
-
货号：
31627 -
别名：
G9A; BAT8; GAT8; NG36; KMT1C; C6orf30 -
应用：
IHC,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q96KQ7 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to EHMT2
Description |
|---|
This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants. |
References |
|---|
| 1.Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):7077-7082. 2.Mol Med Rep. 2016 Nov;14(5):4613-4621. |
Specification |
|
|---|---|
| Aliases | G9A; BAT8; GAT8; NG36; KMT1C; C6orf30 |
| Entrez GeneID | 10919 |
| Swissprot | Q96KQ7 |
| clone | 2D12A11 |
| WB Predicted band size | 132.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human EHMT2 (AA: 317-471) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Immunofluorescence analysis of Hela cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
- Immunofluorescence analysis of Hela cells using EHMT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of HL-60 cells using EHMT2 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using EHMT2 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using EHMT2 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31627
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