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Western blot analysis of Rb (Phospho-Thr356) in HL-60, U2OS, C6 lysates using Phospho-Rb (Thr356) antibody.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Rb (Phospho-Thr356) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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Rabbit Monoclonal Antibody to Phospho-Rb (Thr356)

货号：
P20228
别名：
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
产品上架时间：
2024-01-01
应用：
WB,IP,IHC
反应种属：
Human,Rat
抗体类型：
Primary antibody
Swissprot：
P06400
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Rabbit Monoclonal Antibody to Phospho-Rb (Thr356)

Description
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Description

Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.

Specification
Aliases	RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Entrez GeneID	5925
Swissprot	P06400
WB Predicted band size	Calculated MW: 106 kDa; Observed MW: 110 kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Rat
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Thr356 of human Rb
Formulation	Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.