Rabbit Monoclonal Antibody to Phospho-Histone H3 (Ser10/Thr11)

Description
H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Specification
Aliases	H3 histone; family 3A; H3 histone; family 3B (H3.3B); H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3
Entrez GeneID	8350
Swissprot	P68431
WB Predicted band size	Calculated MW: 15 kDa; Observed MW: 15 kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser10/Thr11 of human Histone H3
Formulation	Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application
WB	1/500-1/1000
IP	1/10-1/20
IHC	1/50-1/100
IF	1/50-1/200