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Western blot analysis of Cdk1/2 (Phospho-Thr14) in HepG2, U2OS, U251 lysates using Phospho-CDK1/2 (Thr14) antibody.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Cdk1/2 (Phospho-Thr14) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Rabbit Monoclonal Antibody to Phospho-CDK1/2 (Thr14)

  • 货号:

    P20165
  • 别名:

    CDKN2; p33(CDK2)
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IP,IHC
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P06493/P24941
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal Antibody to Phospho-CDK1/2 (Thr14)

Description

Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.

Specification

Aliases CDKN2; p33(CDK2)
Entrez GeneID 983/1017
Swissprot P06493/P24941
WB Predicted band size Calculated MW: 34 kDa; Observed MW: 34 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen A synthetic phosphopeptide corresponding to residues surrounding Thr14 of human Cdk1/2
Formulation Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IP 1/10-1/20
IHC 1/50-1/100

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P20165