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Mouse Monoclonal Antibody to ATG5
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货号:
31270 -
别名:
ASP; APG5; APG5L; hAPG5; APG5-LIKE -
应用:
WB,IF,FCM -
反应种属:
Human -
抗体类型:
Primary antibody -
Swissprot:
Q9H1Y0 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180

Mouse Monoclonal Antibody to ATG5
Description |
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The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. |
References |
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1.PLoS One. 2014 Oct 17;9(10):e110293. 2.Sci Transl Med. 2013 Sep 11;5(202):202ra123. |
Specification |
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Aliases | ASP; APG5; APG5L; hAPG5; APG5-LIKE |
Entrez GeneID | 9474 |
Swissprot | Q9H1Y0 |
clone | 3C7A8 |
WB Predicted band size | 32.4kDa |
Host/Isotype | Mouse IgG2b |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human ATG5 (AA: 144-275) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
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IF | 1/100 - 1/500 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using ATG5 mouse mAb against K562 (1), SH-SY5Y (2), and HCT116 (3) cell lysate.
- Immunofluorescence analysis of Hela cells using ATG5 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
- Immunofluorescence analysis of Hela cells using ATG5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using ATG5 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31270

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