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Mouse Monoclonal Antibody to CCND1
-
货号:
31137 -
别名:
BCL1; PRAD1; U21B31; D11S287E -
产品上架时间:
2024-01-01 -
应用:
IHC,ICC,FCM -
反应种属:
Human -
抗体类型:
Primary antibody -
Swissprot:
P24385 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180
Mouse Monoclonal Antibody to CCND1
Description |
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The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. |
References |
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1.BMC Cancer. 2015 Apr 11;15:262. 2.Tumour Biol. 2015 Aug;36(8):6533-40. |
Specification |
|
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Aliases | BCL1; PRAD1; U21B31; D11S287E |
Entrez GeneID | 595 |
Swissprot | P24385 |
clone | 3B6G3 |
WB Predicted band size | 33.7kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human CCND1 (AA: 167-295) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
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IHC | 1/200 - 1/1000 |
ICC | 1/100 - 1/500 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Immunofluorescence analysis of Hela cells using CCND1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of MCF-7 cells using CCND1 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CCND1 mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31137
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