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Mouse Monoclonal Antibody to ANAPC10
-
货号:
31140 -
别名:
DOC1; APC10 -
产品上架时间:
2024-01-01 -
应用:
WB,IHC,IF,FCM -
反应种属:
Human -
抗体类型:
Primary antibody -
Swissprot:
Q9UM13 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180
Mouse Monoclonal Antibody to ANAPC10
Description |
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ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]). |
References |
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1.BMC Cell Biol. 2004 May 16;5:20. 2.Nat Struct Biol. 2001 Sep;8(9):784-8. |
Specification |
|
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Aliases | DOC1; APC10 |
Entrez GeneID | 10393 |
Swissprot | Q9UM13 |
clone | 8F1D10 |
WB Predicted band size | 21.3kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human ANAPC10 (AA: 1-185) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
---|---|
WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
IF | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using ANAPC10 mouse mAb against Hela (1), MCF-7 (2), SK-Br-3 (3), A431 (4), HEK293 (5), A549 (6), and SPC-A-1 (7) cell lysate.
- Immunofluorescence analysis of Hela cells using ANAPC10 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
- Immunofluorescence analysis of Hela cells using ANAPC10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using ANAPC10 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using ANAPC10 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ANAPC10 mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31140
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