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Mouse Monoclonal Antibody to PSMC3
-
货号:
31040 -
别名:
TBP1 -
产品上架时间:
2024-01-01 -
应用:
WB,IHC,ICC,FCM -
反应种属:
Human,Rat,Monkey -
抗体类型:
Primary antibody -
Swissprot:
P17980 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180
Mouse Monoclonal Antibody to PSMC3
Description |
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The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. |
References |
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1.Oncogene. 2012 Apr 5;31(14):1817-24. 2.PLoS One. 2011;6(10):e22800. |
Specification |
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Aliases | TBP1 |
Entrez GeneID | 5702 |
Swissprot | P17980 |
clone | 1G10B7 |
WB Predicted band size | 49kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Rat,Monkey |
Immunogen | Purified recombinant fragment of human PSMC3 (AA: 53-152) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
ICC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using PSMC3 mouse mAb against COS7 (1), C6 (2), Hela (3), and A431 (4) cell lysate.;COS7,C6,Hela,A431
- Immunofluorescence analysis of MCF-7 cells using PSMC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using PSMC3 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PSMC3 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using PSMC3 mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31040
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