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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate.
Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using PDPK1 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to PDPK1
-
货号：
30805 -
别名：
PDK1; PDPK2; PDPK2P; PRO0461 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human,Mouse,Monkey,Rat -
抗体类型：
Primary antibody -
Swissprot：
O15530 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to PDPK1
Description |
|---|
Phosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt, ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main effector of PDK1. |
References |
|---|
| 1.Asian Pac J Cancer Prev. 2012;13(8):4147-51. 2.Mol Cancer Res. 2010 Mar;8(3):421-32. |
Specification |
|
|---|---|
| Aliases | PDK1; PDPK2; PDPK2P; PRO0461 |
| Entrez GeneID | 5170 |
| Swissprot | O15530 |
| clone | 7E4G11 |
| WB Predicted band size | 63.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Monkey,Rat |
| Immunogen | Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF/ICC | 1/50 - 1/200 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate.
- Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using PDPK1 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30805
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