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Mouse Monoclonal Antibody to PDPK1
-
货号:
30806 -
别名:
PDK1; PDPK2; PDPK2P; PRO0461 -
产品上架时间:
2024-01-01 -
应用:
WB,IF,FCM -
反应种属:
Human -
抗体类型:
Primary antibody -
Swissprot:
O15530 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180
Mouse Monoclonal Antibody to PDPK1
Description |
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Phosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt, ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main effector of PDK1. |
References |
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1.Asian Pac J Cancer Prev. 2012;13(8):4147-51. 2.Mol Cancer Res. 2010 Mar;8(3):421-32. |
Specification |
|
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Aliases | PDK1; PDPK2; PDPK2P; PRO0461 |
Entrez GeneID | 5170 |
Swissprot | O15530 |
clone | 3H3D9 |
WB Predicted band size | 63.2kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human PDPK1 (AA: 457-556 expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
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WB | 1/500 - 1/2000 |
IF | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), and U937 (3) cell lysate.
- Immunofluorescence analysis of Hela cells using PDPK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using PDPK1 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30806
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