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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using TUBB1 mouse mAb against K562 (1), HepG2 (2), A431 (3), Jurkat (4), Hela (5), NIH/3T3 (6), Cos7 (7) and PC12 (8) cell lysate.
Immunofluorescence analysis of Hela cells using TUBB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of A431 cells using TUBB1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using TUBB1 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to TUBB1

  • 货号:

    30762
  • 别名:

    Tubulin beta-1 chain
  • 产品上架时间:

    2024-04-01
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human,Mouse,Monkey,Rat
  • 抗体类型:

    Loading Control Antibody
  • Swissprot:

    Q9H4B7
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1320

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Mouse Monoclonal Antibody to TUBB1

Description

This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.

References

Cancer Res. 2012 Sep 15;72(18):4744-52.
Cytoskeleton (Hoboken). 2011 Mar;68(3):175-87.

Specification

Aliases Tubulin beta-1 chain
Entrez GeneID 81027
Swissprot Q9H4B7
clone 2A1A9
WB Predicted band size 50.3kDa
Host/Isotype Mouse IgG1
Antibody Type Loading Control Antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Monkey,Rat
Immunogen Purified recombinant fragment of human TUBB1 (AA: 33-166) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000