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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using PDK2 mouse mAb against Jurkat (1), C6 (2), Cos7 (3), K562 (4), A431 (5) cell lysate.
Flow cytometric analysis of A431 cells using PDK2 mouse mAb (green) and negative control (red).
Mouse Monoclonal Antibody to PDK2
-
货号：
30710 -
别名：
PDHK2; PDKII -
应用：
WB,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q15119 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to PDK2
Description |
|---|
This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
References |
|---|
| 1. J Biol Chem. 2006 May 5;281(18):12568-79 2. Biochemistry. 2004 Oct 26;43(42):13442-51. |
Specification |
|
|---|---|
| Aliases | PDHK2; PDKII |
| Entrez GeneID | 5164 |
| Swissprot | Q15119 |
| clone | 3F2D7 |
| WB Predicted band size | 46.2kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDK2 (AA: 178-404) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using PDK2 mouse mAb against Jurkat (1), C6 (2), Cos7 (3), K562 (4), A431 (5) cell lysate.
- Flow cytometric analysis of A431 cells using PDK2 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30710
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