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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.
Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human kidney tissues using HPRT1 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to HPRT1
-
货号：
30677 -
别名：
HPRT; HGPRT -
应用：
WB,IHC,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P00492 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to HPRT1
Description |
|---|
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout. |
References |
|---|
| 1. Nucleosides Nucleotides Nucleic Acids. 2011 Dec;30(12):1248-55. 2. Mol Ther. 2010 Jan;18(1):54-62. |
Specification |
|
|---|---|
| Aliases | HPRT; HGPRT |
| Entrez GeneID | 3251 |
| Swissprot | P00492 |
| clone | 5F11A7 |
| WB Predicted band size | 24.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HPRT1 (AA: FULL(1-218)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.
- Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human kidney tissues using HPRT1 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30677
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