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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using MAP2K5 mouse mAb against Jurkat (1), A431 (2), A549 (3) cell lysate.
Flow cytometric analysis of Jurkat cells using MAP2K5 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using MAP2K5 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to MAP2K5
-
货号：
30650 -
别名：
MEK5; MAPKK5; PRKMK5; HsT17454 -
应用：
WB,IHC,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q13163 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to MAP2K5
Description |
|---|
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. |
References |
|---|
| 1. DNA Cell Biol. 2012 Mar;31(3):342-9. 2. Wei Sheng Yan Jiu. 2006 Mar;35(2):184-6. |
Specification |
|
|---|---|
| Aliases | MEK5; MAPKK5; PRKMK5; HsT17454 |
| Entrez GeneID | 5607 |
| Swissprot | Q13163 |
| clone | 5H11B10 |
| WB Predicted band size | 50kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MAP2K5 (AA: 63-180) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using MAP2K5 mouse mAb against Jurkat (1), A431 (2), A549 (3) cell lysate.
- Flow cytometric analysis of Jurkat cells using MAP2K5 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using MAP2K5 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30650
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