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Rabbit Polyclonal Antibody to ACTA1/Alpha-actin
-
货号:
P31531 -
别名:
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA -
应用:
WB,IHC,IF,FCM -
反应种属:
Human, Mouse, Rat -
抗体类型:
Primary antibody -
Swissprot:
P68133 -
规格:
-
数量:
-+ -
说明书:
目录价¥1980

Rabbit Polyclonal Antibody to ACTA1/Alpha-actin
Description |
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The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. |
Specification |
|
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Aliases | Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA |
Entrez GeneID | 58 |
Swissprot | P68133 |
WB Predicted band size | 42.1kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the C-terminal region of human ACTA1/Alpha-actin. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
---|---|
WB | 1/1000 |
IHC | 1/100-1/500 |
IF | 1/10-1/50 |
FCM | 1/10-1/50 |
Product Image
- ACTA1/α-actin Antibody (C-term) (Cat. #P31531) western blot analysis in A549,RD cell line and human placenta lysates (35ug/lane).This demonstrates the ACTA1/α-actin antibody detected the ACTA1/α-actin protein (arrow).
- Confocal immunofluorescent analysis of ACTA1/α-actin Antibody (C-term)(Cat#P31531) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- ACTA1/?-actin Antibody (C-term) (Cat. #P31531) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- ACTA1/?-actin Antibody (C-term) (P31531)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1/?-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P31531

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