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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.
Flow cytometric analysis of Jurkat cells using CD14 mouse mAb (green) and negative control (red).
Flow cytometric analysis of COS7 cells using CD14 mouse mAb (green) and negative control (red).
Flow cytometric analysis of Hela cells using CD14 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using CD14 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse kidney using CD14 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat kidney using CD14 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit kidney using CD14 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to CD14
-
货号：
30590 -
别名：
-
应用：
WB,IHC,FCM -
反应种属：
Human,Mouse,Rat,Rabbit -
抗体类型：
Primary antibody -
Swissprot：
P08571 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to CD14
Description |
|---|
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. |
References |
|---|
| 1. J Immunol. 2012 Dec 15;189(12):5729-44. 2. Iran J Immunol. 2011 Jun;8(2):111-9. |
Specification |
|
|---|---|
| Entrez GeneID | 929 |
| Swissprot | P08571 |
| clone | 4B4F12 |
| WB Predicted band size | 40kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Immunogen | Purified recombinant fragment of human CD14 (AA: 20-214) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using CD14 mouse mAb against HepG2 (1), A549 (2), HL60 (3), RAW264.7 (4), Hela (5), HEK293 (6) and NIH/3T3 (7) cell lysate.
- Flow cytometric analysis of Jurkat cells using CD14 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of COS7 cells using CD14 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of Hela cells using CD14 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using CD14 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded Mouse kidney using CD14 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded Rat kidney using CD14 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded Rabbit kidney using CD14 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30590
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