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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using EGFR mutant mouse mAb against SPC-A-1 (1), A549 (2), HepG2 (3) and MCF-7 (4) cell lysate.
Flow cytometric analysis of HepG2 cells using EGFR mutant mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using EGFR mutant mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using EGFR mutant mouse mAb with DAB staining.

Mouse Monoclonal Antibody to EGFR mutant

  • 货号:

    30486P
  • 别名:

    ERBB; HER1; mENA; ERBB1; PIG61
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P00533
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to EGFR mutant

Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

References

1. J Immunol. 2012 Dec 1;189(11):5230-9.
2. J Biol Chem. 2012 Oct 12;287(42):35201-11.

Specification

Aliases ERBB; HER1; mENA; ERBB1; PIG61
Entrez GeneID 1956
Swissprot P00533
clone 5G9B5
WB Predicted band size 175kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000