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Dot blot analysis of anti-Phospho-SMAD4-T277 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with λ phosphatase, using Phospho-SMAD4 Antibody (T277)(upper) or GAPDH (lower).
Confocal immunofluorescent analysis of Phospho-SMAD4-T277 Antibody(Cat#P30236) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded Breast Carcinoma reacted with SMAD4-T277 (center) (Cat.#P30236), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to Phospho-SMAD4(T277)
-
货号：
P30236 -
别名：
Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4 -
应用：
WB,IHC,IF -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
Q13485 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Polyclonal Antibody to Phospho-SMAD4(T277)
Description |
|---|
Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor. |
Specification |
|
|---|---|
| Aliases | Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4 |
| Entrez GeneID | 4089 |
| Swissprot | Q13485 |
| WB Predicted band size | 60.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SMAD4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T277 of human SMAD4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | DB: 1/500 |
| IHC | 1/100-1/500 |
| IF | 1/10-1/50 |
Product Image
- Dot blot analysis of anti-Phospho-SMAD4-T277 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
- Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with λ phosphatase, using Phospho-SMAD4 Antibody (T277)(upper) or GAPDH (lower).
- Confocal immunofluorescent analysis of Phospho-SMAD4-T277 Antibody(Cat#P30236) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Formalin-fixed and paraffin-embedded Breast Carcinoma reacted with SMAD4-T277 (center) (Cat.#P30236), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P30236
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