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Dot blot analysis of anti-Phospho-MYT1-T495 Antibody (Cat. #P30151) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
The anti-Phospho-MYT1-T495 Antibody (Cat. #P30151) is used in Western blot to detect Phospho-MYT1-T495 in HL60 (left) and SK-BR-3 (right) tissue lysates.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Rabbit Polyclonal Antibody to Phospho-MYT1(T495)

  • 货号:

    P30151
  • 别名:

    Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, PKMYT1, MYT1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q99640
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Polyclonal Antibody to Phospho-MYT1(T495)

Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Specification

Aliases Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, PKMYT1, MYT1
Entrez GeneID 9088
Swissprot Q99640
WB Predicted band size 54.2kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This MYT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T495 of human MYT1.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IHC 1/100-1/500