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Rabbit Polyclonal Antibody to Raf1
-
货号:
P23584 -
别名:
RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1 -
应用:
WB,IHC,IF -
反应种属:
Human,Mouse,Rat -
抗体类型:
Primary antibody -
Swissprot:
P04049 -
规格:
-
数量:
-+ -
说明书:
目录价¥1980

Rabbit Polyclonal Antibody to Raf1
Description |
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Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. |
Specification |
|
---|---|
Aliases | RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1 |
Entrez GeneID | 5894 |
Swissprot | P04049 |
WB Predicted band size | Calculated MW: 73 kDa; Observed MW: 73 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300 |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
Application |
|
---|---|
WB | 1/500-1/1000 |
IHC | 1/50-1/100 |
IF | 1/50-1/200 |
ELISA | 1/10000 |
Product Image
- Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody.
- Western blot analysis of Raf1 in rat liver lysates using Raf1 antibody. The lane on the right is blocked with the synthesized peptide.
- Immunofluorescence analysis of Raf1 in mouse liver using Raf1 antibody(red),and DAPI (blue).
- Immunohistochemistry analysis of paraffin-embedded rat kidney tissue using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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