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Western blot analysis of Raf1 in HepG2 lysates using Raf1 antibody.
Western blot analysis of Raf1 in rat liver lysates using Raf1 antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of Raf1 in mouse liver using Raf1 antibody(red),and DAPI (blue).
Immunohistochemistry analysis of paraffin-embedded rat kidney tissue using Raf1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Rabbit Polyclonal Antibody to Raf1

  • 货号:

    P23584
  • 别名:

    RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P04049
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to Raf1

Description

Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.

Specification

Aliases RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Entrez GeneID 5894
Swissprot P04049
WB Predicted band size Calculated MW: 73 kDa; Observed MW: 73 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen The antiserum was produced against synthesized peptide derived from human C-RAF. AA range:251-300
Formulation Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IHC 1/50-1/100
IF 1/50-1/200
ELISA 1/10000