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Western blot analysis of Rho A + B + C expression in (1) Jurkat lysates; (2) NIH/3T3 lysates using RhoA/B/C antibody
Western blot analysis of Rho A + B + C in SH-SY5Y and rat Brain lysates using Rho A + B + C antibody.
Immunofluorescent analysis of RhoA/B/C in Hela using RhoA/B/C antibody.
Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma using Rho A + B + C antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Rabbit Polyclonal Antibody to RhoA/B/C

  • 货号:

    P23440
  • 别名:

    ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P61586/P62745/P08134
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Polyclonal Antibody to RhoA/B/C

Description

RhoA,RhoB,RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Specification

Aliases ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC
Entrez GeneID 387/388/389
Swissprot P61586/P62745/P08134
WB Predicted band size Calculated MW: 22 kDa; Observed MW: 22 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen A synthesized peptide derived from human Rho A + B + C
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500-1/1000
IHC 1/50-1/100
IF 1/50-1/200
FCM 1/50-1/100