PHB[30264]
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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western blot analysis using PHB mouse mAb against A431 (1), MCF-7 (2), Jurkat (3), Hela (4), HepG2 (5), A549 (6), NIH/3T3 (7), Cos7 (8) and PC-12 (9) cell lysate.
Immunofluorescence analysis of NIH/3T3 cells using PHB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of MCF-7 cells using PHB mouse mAb (blue) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using PHB mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues using PHB mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PHB mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PHB mouse mAb with DAB staining.

Mouse Monoclonal Antibody to PHB

  • 货号:

    30264
  • 别名:

    PHB1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human,Mouse,Rat,Monkey
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P35232
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to PHB

Description

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

References

1. Biochem Biophys Res Commun. 2009 Dec 18;390(3):1023-8.
2. J Cell Biochem. 2009 Nov 1;108(4):926-34.

Specification

Aliases PHB1
Entrez GeneID 5245
Swissprot P35232
clone 5H7
WB Predicted band size 30kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat,Monkey
Immunogen Purified recombinant fragment of human PHB expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000