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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western blot analysis using EEF2 mouse mAb against HepG2 (1), Hela (2), HEK293 (3) and A431 (4) cell lysate.
Immunofluorescence analysis of 3T3-L1 cells using EEF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (left) and kidney cancer tissues (right) using EEF2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues (left) and tonsil tissues (right) using EEF2 mouse mAb with DAB staining.
Mouse Monoclonal Antibody to EEF2
-
货号：
30190 -
别名：
EF2; EEF-2; EEF2 -
应用：
WB,IHC,IF -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P13639 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to EEF2
Description |
|---|
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. (provided by RefSeq) |
References |
|---|
| 1. Mol Cell Biol. 2008 Dec;28(23):7050-65. 2. Am J Physiol Regul Integr Comp Physiol. 2009 Feb;296(2):R326-33. |
Specification |
|
|---|---|
| Aliases | EF2; EEF-2; EEF2 |
| Entrez GeneID | 1938 |
| Swissprot | P13639 |
| clone | 5B6 |
| WB Predicted band size | 95kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human EEF2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| IF/ICC | 1/200 - 1/1000 |
| ELISA | 1/10000 |
Product Image
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
- Western blot analysis using EEF2 mouse mAb against HepG2 (1), Hela (2), HEK293 (3) and A431 (4) cell lysate.
- Immunofluorescence analysis of 3T3-L1 cells using EEF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissues (left) and kidney cancer tissues (right) using EEF2 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues (left) and tonsil tissues (right) using EEF2 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30190
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