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Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western blot analysis using BECN1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.
Flow cytometric analysis of RAJI cells using BECN1 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and liver cancer tissues (right) using BECN1 mouse mAb with DAB staining.
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Mouse Monoclonal Antibody to BECN1
-
货号：
30182 -
别名：
ATG6; VPS30; beclin1; BECN1 -
应用：
WB,IHC,FCM -
反应种属：
Human,Mouse -
抗体类型：
Primary antibody -
Swissprot：
Q14457 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to BECN1
Description |
|---|
Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 (PubMed 19270693)).(supplied by OMIM) . Tissue specificity: Ubiquitous. |
References |
|---|
| 1. Autophagy. 2008 Oct 1;4(7):947-8. 2. J Clin Invest. 2008 Jun;118(6):2190-9. |
Specification |
|
|---|---|
| Aliases | ATG6; VPS30; beclin1; BECN1 |
| Entrez GeneID | 8678 |
| Swissprot | Q14457 |
| clone | 2A4 |
| WB Predicted band size | 60kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human BECN1 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
- Western blot analysis using BECN1 mouse mAb against Hela (1), A431 (2), MCF-7 (3), RAJI (4), Jurkat (5) and SKBR-3 (6) cell lysate.
- Flow cytometric analysis of RAJI cells using BECN1 mouse mAb (green) and negative control (purple).
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and liver cancer tissues (right) using BECN1 mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30182
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