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        Mouse Monoclonal Antibody to SMAD5
- 
										
货号:
30150 - 
										
别名:
Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5 - 
										
应用:
WB,IHC,IF,FCM - 
										
反应种属:
Human,Rat - 
										
抗体类型:
Primary antibody - 
										
Swissprot:
Q99717 - 
										
规格:
 - 
										
数量:
-+ - 
										
										
说明书:
目录价¥2180
 
						        
						        
						        
						        
						    Mouse Monoclonal Antibody to SMAD5
Description | 
									
|---|
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous. | 
									
References | 
									
|---|
| 1. Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3927-32.   2. Nat Cell Biol. 2008 May;10(5):567-74.  | 
									
Specification | 
									|
|---|---|
| Aliases | Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5 | 
| Entrez GeneID | 4090 | 
| Swissprot | Q99717 | 
| clone | 3H9 | 
| WB Predicted band size | 52kDa | 
| Host/Isotype | Mouse IgG1 | 
| Antibody Type | Primary antibody | 
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. | 
| Species Reactivity | Human,Rat | 
| Immunogen | Purified recombinant fragment of human SMAD5 expressed in E. Coli. | 
| Formulation | Purified antibody in PBS with 0.05% sodium azide. | 
Application | 
									|
|---|---|
| WB | 1/500 - 1/2000 | 
| IHC | 1/200 - 1/1000 | 
| IF/ICC | 1/200 - 1/1000 | 
| FCM | 1/200 - 1/400 | 
| ELISA | 1/10000 | 
Product Image
- Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
 - Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
 - Flow cytometric analysis of Jurkat cells using SMAD5 mouse mAb (green) and negative control (purple).
 - Immunohistochemical analysis of paraffin-embedded human brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.
 
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30150
						
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