Rabbit Monoclonal Antibody to RPA32/RPA2

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single~stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co~ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3~related protein (ATR) kinase. The RPA complex protects single~stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co~ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low~affinity and high~affinity mode, determined by which oligonucleotide/oligosaccharide~binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high~affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post~translational modifications of the RPA complex also plays a role in co~ordinating different damage response pathways. [provided by RefSeq, Sep 2017]