Rabbit Monoclonal Antibody to IDH1

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2~oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)~dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)~dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)~dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)~dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS~1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4~dienoyl~CoAs to 3~enoyl~CoAs, as well as in peroxisomal reactions that consume 2~oxoglutarate, namely the alpha~hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]