Rabbit Monoclonal Antibody to APAF1

APAF Oligomeric Apaf~1 mediates the cytochrome c~dependent autocatalytic activation of pro~caspase~9 (Apaf~3), leading to the activation of caspase~3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Induced by E2F and p53 in apoptotic neurons. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf~1 and pro~caspase~9 bind to each other via their respective NH2~terminal CARD domains and consecutively mature caspase~9 is released from the complex. Pro~caspase~3 is recruited into the Apaf~1~pro~caspase~9 complex via interaction with pro~caspase~9. Interacts with APIP. 6 isoforms of the human protein are produced by alternative splicing.