Rabbit polyclonal antibody to Rev~Erbα

Reverse orientation c~erbA gene α (Rev~erbα, EAR~1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins. Rev~erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev~erbα potentiates the adipocyte differentiation of 3T3~L1 cells. In addition, expression oscillates with circadian rhythm in liver cells and Rev~erbα regulates expression of BMAL1, ApoA~I and ApoC~III, all key regulators of circadian rhythm. Phosphorylation of Rev~erbα Ser55 and Ser59 by GSK~3β appears to stabilize Rev~erbα protein levels and is important for synchronizing and maintaining the circadian clock. Rev~erbα also regulates inflammation by targeting the NF~κB responsive genes IL~6 and COX~2. Rev~erbα lacks the activation function 2 domain required for ligand~dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co~repressor (N~CoR)/HDAC3 complex to target genes to repress transcription.