Rabbit polyclonal antibody to BID

Bid is a pro~apoptotic “BH3 domain~only” member of the Bcl~2 family originally discovered to interact with both the anti~apoptotic family member Bcl~2 and the pro~apoptotic protein Bax. Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase~8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane. Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf~1 and caspase~9 and resulting in caspase~9 activation. Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation.