Rabbit polyclonal antibody to AKT

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK~β) and Akt 3 (also designated PKBγ or thyoma viral proto~oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c~Akt proto~oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR~β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3~kinase (PI 3~kinase) complex. Activation of Akt1 by insulin or insulin~growth factor~1(IGF~1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF~1~stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.