Rabbit polyclonal antibody to IL~10Rα (phospho~Y496)

The IL~10 receptor, IL~10R, is a member of the class II subgroup of the cytokine receptor family and exhibits structural similarity to the interferon receptor. IL~10R is expressed in B cells and T helper cells, as well as in LPS~induced mouse fibroblasts. Overall, mouse IL~10R and human IL~10R share 60% sequence identity at the protein level. Stimulation with IL~10 leads to phosphorylation of JAK1 and Tyk 2 tyrosine kinases. The activated kinases phosphorylate the two tyrosine residues (Tyr 446 and Tyr 496) in the cytoplasmic domain of IL~10Rα. The phosphorylation of these two residues are required for proper function of IL~10R and activation of IL~10E1 signaling. IL~10Rβ is ubiquitously expressed and, in addition to forming the IL~10 heterodimeric receptor, it forms a heterodimeric receptor with an IL~22R subunit and an IL~28R subunit. IL~10R is constitutively expressed on human natural killer (NK) cells and the direct binding of IL~10 potentiates cytokine production by human NK cells.